Journal: Molecular Psychiatry

Article Title: Heavy adolescent drinking makes the adult brain more vulnerable to ethanol by permanently altering the age-dependent interplay between alcohol, GIRK channels and activin

doi: 10.1038/s41380-025-03210-x

Figure Lengend Snippet: (a-b) In whole-cell voltage-clamp recordings (V h −70 mV) from GCs in brain slices of adolescent (a ) and adult mice ( b ), increasing EtOH concentrations induced outward currents in a reversible, dose-dependent fashion. Recordings were made in slices from wt mice (black traces) and from dnActRIB mice (red traces), and in wt slices incubated with recombinant activin A (3–6 h, 25 ng/ml, blue traces). Scale bars: 2 min, 20 pA. ( c-d ) Dose-responses curves show outward current amplitude as function of EtOH dose for the recording conditions illustrated above. ( e ) Comparison of EtOH-induced outward current in GCs from adult wt mice recorded in TTX alone (left column) or in combination with blockers of ionotropic GABA and glutamate receptors (picrotoxin, PTX, 100 µM; kynurenic acid, KA, 2 mM, middle column), or without any blocker (right column). ( f-g) Columns show hippocampal weight in adolescence and adulthood ( f ), and the normalized levels of activin A protein in hippocampus as determined by ELISA in the two age groups ( g ). Statistical comparisons were performed using one- or two-way ANOVA followed by Tukey’s post-hoc test ( c-e ) or two-tailed student’s t-test ( f-g ). * p < 0.05; ** p < 0.01; *** p < 0.001.

Article Snippet: To investigate the effect of activin on GIRK channel activity, slices were incubated with recombinant activin A (25 ng/ml; R&D System, Minneapolis, MN, USA).

Techniques: Incubation, Recombinant, Comparison, Enzyme-linked Immunosorbent Assay, Two Tailed Test

Journal: Molecular Psychiatry

Article Title: Heavy adolescent drinking makes the adult brain more vulnerable to ethanol by permanently altering the age-dependent interplay between alcohol, GIRK channels and activin

doi: 10.1038/s41380-025-03210-x

Figure Lengend Snippet: ( a ) Dose-response curves demonstrate augmenting effect of DID on acute EtOH application in GCs from adult mice of either genotype. Incubating wt-DID slices with activin A (25 ng/ml for 3–6 h) further enlarged EtOH response. Traces above depict outward current responses to increasing EtOH concentrations in GCs from adult wt mice with adolescent SH (black trace) or DID experience (blue trace). (b) ELISA measurements reveal higher activin A protein levels in hippocampi from DID-exposed adult wt and dnActRIB mice adults compared to alcohol-naive littermates. (c) Histograms summarize tonic Ba 2+ - and TQ-sensitive currents in GCs from the different groups. ( d ) Histograms summarize TQ-sensitive fraction of total EtOH-evoked current in GCs from the groups as indicated by like-colored letters above columns. Ba 2+ fully suppressed EtOH response in all groups. (e) Traces depict GIRK current activation by additive application of baclofen (0.3 µM) and EtOH (80 mM EtOH) in GCs from wt control (SH, upper trace) and wt DID mouse (lower trace). Histogram on right shows effect of baclofen alone. ( f ) Histograms compare GIRK current response to EtOH alone or in the presence of pre-applied baclofen. Statistical comparisons were performed using an unpaired, two-tailed student’s t-test at α = 0.05 ( b, c, e, f ) or a one or two-way ANOVA followed by Tukey’s post-hoc test ( a, d ). * p < 0.05; ** p < 0.01; *** p < 0.001.

Article Snippet: To investigate the effect of activin on GIRK channel activity, slices were incubated with recombinant activin A (25 ng/ml; R&D System, Minneapolis, MN, USA).

Techniques: Enzyme-linked Immunosorbent Assay, Activation Assay, Control, Two Tailed Test

Journal: Cell Reports Medicine

Article Title: ACVR2A attenuation impacts lactate production and hyperglycolytic conditions attracting regulatory T cells in hepatocellular carcinoma

doi: 10.1016/j.xcrm.2025.102038

Figure Lengend Snippet:

Article Snippet: recombinant human/mouse/rat activin A PLUS protein , MBL , Cat# QK005-0100.

Techniques: Staining, Purification, Recombinant, Modification, Transfection, Functional Assay, Antibody Labeling, Lactate Assay, Glucose Assay, Blocking Assay, Western Blot, Reverse Transcription, Cell Culture, Bicinchoninic Acid Protein Assay, RNA Sequencing, Gene Expression, Plasmid Preparation, shRNA, Software, Real-time Polymerase Chain Reaction, Filtration